Description
Advanced – For more experienced classes; requires some technical skill.
This kit module is great for teaching:
- The molecular basis of heredity
- The relationship between genes and proteins
- How E. coli is used to engineer proteins useful in medicine and manufacturing
- Three techniques – transformation, protein purification, and protein gel electrophoresis – commonly used in biological research and its areas of application
This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 3 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).
In this activity students take their successfully purified GFP and run a polyacrylamide gel to determine the results of their purification technique. Students analyze the band of purified GFP, approximately 27kDa, and compare it to the banding pattern of unpurified cell lysate to determine the effectiveness of their purification process. This activity typically takes 90 minutes for students to prepare, load, and run their gels followed by a COOMASSIE® blue staining process.
Note: Kit includes a voucher to request perishable material later at your convenience. Contact us or return the voucher to request delivery of perishable materials. Refrigerate polyacrylamide gels and protein marker until use.