Description
Advanced – For experienced high school and college classes; requires some technical skill.
This classic lab was developed in cooperation with the DNA Learning Center. Students perform restriction digests on bacteriophage lambda DNA, conduct electrophoretic analysis of the reactions, and stain the resulting gel. They then observe and analyze the stained patterns of fragments separated by size during gel electrophoresis.
Materials are sufficient for 6 teams of students or 6 separate instructor demonstrations. Kit contains perishable materials. Note: Store restriction enzymes in a freezer.