Description
Advanced—For experienced high school and college classes; requires some technical skill.
Generate excitement by having your students amplify their own mitochondrial DNA for sequencing!
- Uses a simple, robust protocol ideal for the classroom
- Enlivens discussions on the molecular basis of heredity and evolution
- Provides a basic version of the lab that is used in forensics and the analysis of ancient DNA samples
- Teaches real-world applications of PCR
- Includes online resources to enhance the learning experience
Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs). After gel electrophoresis confirms amplification, the student samples may be sent for sequencing (the cost of sequencing is not included in the price of the kit). Students then compare their SNPs to ancient hominids and to people from different world populations to discover patterns of DNA variation and to analyze theories of human evolution.
Kit contains instructions and materials for 25 students to perform the DNA extraction and amplification step of the protocol. Kit does not include materials for electrophoresis. Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.