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Owl Pellet Dissection

AYVA was recently given the opportunity to dissect different sizes of  Carolina’s owl pellets! Even with the limited stock, we were able to use both the small and large owl pellets to experiment on.

Owl pellets are remnants of an owl’s diet and digestive process, composed primarily of indigestible parts of prey such as bones, fur and feathers. These pellets are regurgitated several hours after an owl consumes its prey, and the process of regurgitation can last from seconds to several minutes. Young owls do not produce pellets until they have begun to eat their prey whole.

These pellets provided us with a fascinating look on what an owl’s regular feeding diet may look like. Within both pellets we found multiple bones relating to brown rats, one of the many food sources of everyday barn owl’s. These bones included the femur, humerus, tibia, fibula, jaw, skull and pelvic bone. These were easy to identify thanks to the owl pellet study kit. This kit contains a manual with plenty of pictures of all the bones that you might find within an owl pellet.

 

While we only dissected two owl pellets, each pellet can differ from the next, whether they are small or large. The shape and texture of a given owl pellet depends on the species owl and its prey. Pellets can be tightly compacted, oval, and furry, or loosely packed with an irregular shape.

As we compared the dissection of the larger and smaller pellet we noticed that there are more remains in the larger pellet. The larger owl pellet had more identifiable remnants of the prey making it easier to determine the owls’ feeding habits and local ecosystem. By using the larger pellets, students have more components to analyze to give a closer look into the owls behavior.

What Are Owl Pellets?

owl pellets

See what you can learn about birds by studying the pellets they leave behind.

Most birds cannot chew their food, and owls are no exception. Owls usually swallow their prey whole. Owls differ from other species of birds because they do not have a crop, the baglike organ used to store food after it has been swallowed so that it can be digested later. In owls, food passes directly from the mouth to the gizzard. The gizzard is an organ that uses digestive fluids and bits of sand and gravel to grind and dissolve usable tissue from the prey.

The types of tissue that can be dissolved by an owl’s digestive system include muscle, fat, skin, and internal organs. These tissues are broken down into a variety of nutritional substances by the owl’s gizzard and intestines. Some of these tissues (e.g., fur and bones) cannot be digested. The digestible material, along with other waste collected throughout the body, is ejected from the vent, which is the combination reproductive and excretory opening in birds. The pasty white excrement is known as urea. It is rich in nitrogen and similar to urine in mammals, only thicker.

What happens to the indigestible material?

Indigestible material left in the gizzard such as teeth, skulls, claws, and feathers are too dangerous to pass through the rest of the owl’s digestive tract. To safely excrete this material, the owl’s gizzard compacts it into a tight pellet that the owl regurgitates. The regurgitated pellets are known as owl pellets.

Owl pellets are useful to researchers because they can find out quite a bit about an owl’s lifestyle through careful examination of the pellet’s contents. Since most of the prey’s bones are not actually broken during the attack and the subsequent digestion process, they can be readily identified in the pellet. Most pellets include a skull or skulls, which makes identification of the prey relatively simple. If an owl consumes multiple prey in a short period of time, it forms one large pellet from the remains.

Large owls are obviously capable of making large pellets. However, since large owls do not always eat large prey, one cannot always determine the size of the owl that left a given pellet solely based on the size of the pellet. In addition, a startled owl may eject a pellet that is not fully compacted, thereby giving the pellet a larger appearance than normal. Other species of birds such as hawks and eagles produce pellets, but they are smaller and contain fewer animal parts than those produced by owls.

Skulls and other bones can be found during an owl pellet dissection.

Storing and regurgitating pellets

An owl pellet generally reaches its final form a few hours after the owl has eaten. However, the pellet is not usually ejected immediately after it is formed. Owls can store a pellet in a structure known as the proventriculus for as long as 20 hours before disgorging it. Since the stored pellet partially blocks the entrance to the digestive system, it must be ejected before the owl can eat again. Young owls do not produce pellets until they have begun to eat their prey whole.

The actual process of regurgitating a pellet lasts from a few seconds to several minutes. The pellet is forced out by spasms of the owl’s esophagus. These spasms make the owl look like it is coughing painfully. However, it is not hurt by the process because the pellet remains soft and moist until it leaves the owl’s body.

Identifying pellets

The shape and texture of a given owl pellet depends on the species of the owl that produced it and the type of prey that the owl consumed. Some pellets are tightly compacted, oval, and furry. Others are loosely compacted with an irregular shape. Pellets are moist when they are first ejected, but quickly dry out and start to decompose once they leave the owl’s body. Owl pellets are typically found near places where owls perch, such as under trees and near barns.

Barn Owl pellets are typically medium sized, smooth, cylindrical, and dark. The tiny Elf Owl has a very small pellet that is dry and loosely compacted, a result of its largely insect diet. The Great Horned Owl can produce pellets that are 3 to 4 inches long. These pellets are usually cylindrical and tightly compacted. The exterior of the pellet can vary greatly due to the vast array of prey that Great Horned Owls consume.

Owl pellet dissection resources

An owl pellet dissection gives students a glimpse into the life of an animal they may never see in the wild. Pellets tell us what the owl eats, where it is likely to roost, what small mammals live nearby, and even the relative proportions of those animals. Safe owl pellet dissections can build toward several NGSS standards across grade levels.

 

Frequently Asked Questions about Living Cultures

Many of you have questions about ordering and caring for cultures of protozoa, protists, and bacteria. We’ve gathered the most frequently asked questions here, along with the answers, as a quick reference. While it should answer most common questions and concerns, organism-specific information can be found in the instructions shipped with the culture and by searching our Carolina™ Care Sheets. Mitosis

Protozoa and protists

“When should I order protozoa?”

A living culture should be ordered a couple of days before use. We recommend receiving it on a Tuesday, Wednesday, or Thursday and using it the same week if at all possible. Try to arrange delivery at a time when you can personally receive the package and examine its contents. Inform your institution’s receiving department or office personnel to contact you immediately once it arrives. If you can’t get to the package right away, have someone place it in a climate-controlled area—NOT in a refrigerator—until you can attend to it.

“How long will my protozoa survive in the shipping container?”

Protozoa and algae cultures will survive for about one week in the shipping container. When you receive your protozoa culture, remove the lid and gently aerate the culture using a clean pipet. Allow the culture to settle after aeration and then use a stereomicroscope to check for activity. Replace the lid and set the culture in an area away from direct sunlight or extreme temperature changes.

  • For Euglena, make sure the cap is loose and place the container in a well-lit area, but not in direct sunlight.
  • As a rule of thumb, don’t place a protozoa, algae, or bacteria culture in the refrigerator. Exceptions are particular cultures of fungus that should be refrigerated to slow their growth, and when the instructions specify that refrigeration is an acceptable means of storage.

“How can I extend the life of my protozoa if I don’t want to subculture them?”

We recommend using the culture within 5 days of receipt. However, the life of your protozoa can be extended by feeding and by replenishing the culture water. For organisms that feed on bacteria, a single grain of precooked rice can be added to the culture container. Some organisms require algae or other food sources; be certain to research what food the organism requires prior to ordering. To replenish the culture water, allow the organisms to settle to the bottom of the jar, and then gently pour off about a quarter of the water and replace it with fresh springwater. Never use tap or distilled water.

“Some of the water from the culture container spilled (or evaporated) and I want to add more water. Can I just add some tap water? What kind of water is best?”

Never add tap water to a culture. It contains chlorine and in some cases chloramines that are toxic to protozoa, algae, and other living organisms such as fish and tadpoles. Use room temperature springwater instead. We recommend Carolina™ Springwater, which we use to grow many of our organisms. If this is not an option, you can purchase springwater from your local grocery store. Be sure to carefully read the label to ensure there are no additional additives for taste, and that the source is not a municipal water supply. The label should state that the water came from a “natural springwater” source.

“What types of microscopes can be used to view live organisms?”

For protozoa and algae use a compound microscope for close, detailed viewing. To observe swimming and interactions with other organisms within the culture, use a stereomicroscope. Use a compound microscope with an oil-immersion objective to view bacteria and fungus.

“What are the ingredients of Alga-Gro® media?”

Alga-Gro® media are proprietary mixtures; therefore, we do not disclose specific information about the amounts or types of ingredients they contain.

“What is the enzyme concentration?”

Enzymes do not have a specified concentration like chemicals do (e.g., 3% hydrogen peroxide) they have an activity level. The activity level varies with each enzyme. If you need to know the activity level of a particular enzyme, call our Customer Service Department (800.334.5551); and ask the representative to connect you with a technician in our Cultures Department  for this information.

“What types of organisms will I see in the Carolina™ Pond Mystery Mix or the Hay Infusion Kit?”

The number and types of organisms vary from culture to culture. Remember to always use springwater when setting up a culture; do not use tap or distilled water. It takes time for organisms to appear and become abundant, so allow at least one to 2 weeks for organisms to grow and emerge.

How to maintain algae in your classroom:

Do

  • Keep at room temperature (22° C or 72° F)
  • Loosen caps on tubes or jars and keep upright
  • Use sterile culture vessels and pipets
  • Use within 5 days of receipt or set up subcultures after 5 days to maintain longer
  • Provide correct light intensity (indirect sunlight or artificial light)
  • Read the Carolina™ Culturing Algae Manual—included with each order of algae and available separately

Don’t

  • Put in the refrigerator
  • Put in direct sunlight
  • Wash glassware in detergent
  • Keep above 30° C (86° F)—lethal to algae

Bacteria

Do I need to place my bacteria cultures in an incubator when I receive them?” The short answer is no, not every bacteria culture needs to be incubated. The temperature listed on the label is the temperature at which the culture would optimally grow if you were growing it in subculture. Most bacteria store well at room temperature and will remain viable for 3 to 4 weeks. Of course, there’s always the exception! Spirillum volutans needs to be kept at 30° C.

“Can my students get cancer from using Agrobacterium tumefaciens in the Carolina™ Plant Cancer Study Kit?”

No, they can’t. The cancer seen in susceptible plants is the result of a bacterial infection that is specific to plants, not humans. Susceptible plants include roses, grapes, apples, cherries, pecans, sunflowers, tobacco, beets, turnips, and tomatoes.

“Do I have to use a fresh, 24-hour culture of bacteria to perform a Gram stain?” Yes, you do. As the culture ages, the bacterial cell walls become more permeable to the crystal violet-iodine complex, making the cells easily decolorized. This will cause Gram-positive bacteria to stain negative.

How to Handle, Store, and Repair Microscope Slides

Carolina™ prepared microscope slides provide an essential component for the in-depth study of botany, zoology, histology, embryology, parasitology, genetics, and pathology. After receiving your slides, proper care will keep them in good condition and make them last as long as possible. In the following paragraphs, we’ll discuss the handling, storage, and repair of prepared slides.

Handling

Teach students proper slide handling and slides can be used year after year. Slides should be held by the edges, avoiding the cover glass area. Always begin viewing a slide using the microscope’s lowest magnification. This reduces the risk of contact by the microscope’s objective lens. Afterwards, switch to a higher magnification if needed.

Keep the microscope’s objective lens and other objects from coming into contact with a slide. Pressure on the cover glass can cause it to break or loosen. When finished viewing, remove the slide from the microscope and place it in its storage container. Leaving the slide on the illuminated stage for extended periods of time can cause fading and other damage.

When slides get soiled, you can clean them with soapy water or isopropyl alcohol. Do not immerse slides in water or soak them in it. This loosens the cover glass adhesive, causing the cover glass to come off and possibly ruin the slide.

Storage

To keep your prepared microscope slides in good condition, always store them in a container made for the purpose and away from heat and bright light. The ideal storage area is a cool, dark location, such as a closed cabinet in a temperature-controlled room. Stained slides naturally fade over time. Keeping them in a cool, dark location helps slow down the process.

Slides should be kept horizontal (flat) with the specimen side up. If they are stored on edge, the cover glass or specimen may shift out of position. Take care not to stack slides on top of one another or apply pressure to the cover glass.

Repair

Common problems include a broken slide or cover glass, bubbles in the mounting agent, and specimens shifted to the edge of the cover glass. If a slide or cover glass is broken, dispose of it and replace it immediately to prevent anyone from being cut. The adhesive used to attach a cover glass to a slide is applied as a liquid. As the liquid dries, it only hardens around the edges of the cover glass. With rough handling this seal can crack or loosen, allowing the liquid to ooze out. You can fix a broken seal by applying a small amount of fresh mounting media to the break. Clear nail polish sometimes works if you don’t have any mounting media handy.

Most slide repairs require some amount of skill. Often it is easier and more cost effective to replace the slide rather than to repair it.

Carolina Biological Products Named Best-in-Class for K to 12 Science Teaching and Learning in National STEM Awards

Educators Pick Best of STEM® 2023 Awards recognize innovative STEM products for education that go above and beyond to inspire today’s teachers, students and science classes

BURLINGTON, NC, Aug. 30, 2023 – Carolina Biological is proud to announce that its products won a number of new science education awards. Leading K-12 science supplier Carolina Biological Supply Company won four prestigious national Educators Pick Best of STEM® 2023 Awards and earned Finalist in two more categories. The awards are the only competition in which products are reviewed and judged only by STEM educators. Thirty-four awards total were given in thoughtful and important categories tailored to STEM education. The awards program, operated in partnership with the National Science Teaching Association (NSTA), MCH Strategic Data and the National Association of Biology Teachers (NABT), spotlights innovative products, technologies, and services that are changing the world of STEM education.

Winners were chosen for qualities such as alignment with NGSS standards, the use of simulations, instructional support for teachers, and the flexibility of implementation for different modes of instruction across all STEM topics. Carolina Biological Supply Company was awarded for: Building Blocks of Science® 3D won “Bridging the Gap—English Language Learners: Science & Literacy,” Smithsonian Science for the Classroom™ won “Best of STEM: Most Comprehensive Culturally Relevant Teaching,” and Carolina® Healthcare Simulations Kit Series: Blood Typing Kit won “CTE Training: Health Science—Training Kits” and Carolina Distance Learning® Online Gateway HyFlex won a “Trailblazer Award: Hybrid Learning Superhero.” Carolina also received Finalist status for 3-D Earth and Space Science Kits for “Best of STEM: Environmental Science” and CRISPR in a Box™ Kit for “Trailblazer: Biotechnology Innovator.”

Products were judged first by an expert panel of judges. Then, more than 267,000 STEM educators were invited to evaluate the finalists via survey. For information about the awards program, visit www.bestofstemawards.com. Carolina has won eight Educator’s Pick Best of STEM awards over the last three years, demonstrating breadth and depth of high-quality products, curricula and teacher resources in K-16 education. Awards have been earned in such subjects as elementary and middle-school science, biology, chemistry, physical science, environmental science, career technical education (CTE), and anatomy and physiology.

“Carolina employees put their hearts and minds into developing new products to support science teachers at all instructional levels,” said Jim Parrish, CEO of Carolina Biological. “It’s rewarding to have those we care so much about affirm our work and mission with Educators Pick Best of STEM® 2023 Awards.”

“Our development staff works closely with science teachers to develop products that will excite and engage their students,” said Mark Meszaros, Vice President of Carolina Biological’s Core Product Management and Innovation. “I am grateful that we were able to address these difficult to teach topics and be recognized by our peers in science education.”

“This recognition highlights Carolina’s dedication to fostering inclusive and effective learning environments that empower students in science and STEM, said Karen Stevens, Vice President of Carolina Biological’s Curriculum. “This is the second Best of STEM award for Building Blocks of Science® 3D, which reinforces Carolina’s commitment to support all students by ensuring that language barriers do not hinder students’ opportunities to excel. And we’re thrilled that Smithsonian Science for the Classroom™ was recognized for Most Comprehensive Culturally Relevant Teaching. Developed with support from the Smithsonian Institution — the world’s largest cultural institution – culturally relevant teaching is crucial for engaging students from diverse backgrounds and making STEM subjects accessible to all.”

“We are thrilled and honored to receive the Educators Pick Best of STEM® 2023 Awards, ‘Trailblazer Award: Hybrid Learning Superhero’ for our groundbreaking Carolina Distance Learning product for college, Carolina Online Gateway, HyFlex,” said Shannon McGurk, Vice President of Carolina Biological’s Carolina Distance Learning. “This recognition reaffirms our commitment to revolutionizing education through innovation and hybrid learning solutions. Carolina Online Gateway represents a new era of educational tools, providing educators and students with a comprehensive platform designed to elevate the college distance learning experience.”

All four of Carolina’s winning products and the two finalists are available now through Carolina. For information, visit carolina.com or call (800) 334-5551 to speak to a customer service or technical representative.

Carolina’s 2023 Best-in-Class Winners:

The first winner, setting the standard in 3D learning and 3D assessment, is the Smithsonian Science for the Classroom curriculum. It provides cohesive storylines, superior teacher support, and research-supported instructional design that is backed by proven results. Smithsonian Science for the Classroom is a high-quality core science and engineering curriculum program specifically developed to meet the NGSS. This elementary curriculum is designed to engage, inspire, and connect grades K to 5 students firsthand to the world around them through engaging and hands-on lessons.

Next, Carolina Biological’s second winner, Building Blocks of Science 3D provides hands-on unit kits for students in grades K to 5. This complete, phenomena-based program combines interactive investigations, literacy components, and digital resources to teach students science content and investigative skills in manageable 30-minute lessons. The program also integrates engineering into every grade and includes everything teachers need for their entire class for every lesson with hands-on materials, print, and digital simulations.

In addition, the third winner, Carolina® Healthcare Simulations Kit Series: Blood Typing Kit, is a Career Technical Education (CTE) series that asks students to practice clinical skills, techniques, and procedures. While performing a series of investigations, in addition to gaining an understanding of human anatomy and physiology, students learn and practice skills that can be used in a healthcare setting using safe simulated samples. Each activity includes a case study that provides students with background information about a patient who presents with relevant symptoms. The series consists of Blood Hematocrit, Blood Glucose, Blood Typing, Urinalysis, and a Vital Signs Kit. More real-world CTE kits in the series are coming soon.

The fourth winner, Carolina Distance Learning® Online Gateway HyFlex, is for college level and includes lab kits and materials that produce great results for college students, at home or on campus. A HyFlex class allows students to attend class in person, synchronously online, or asynchronously online. That flexibility gives students the opportunity to attend class who, due to circumstances, might not otherwise be able to do so. It also gives institutions the opportunity to increase enrollment and the number of courses they can offer.

Which Electrophoresis Kit Is Right for You?

Use this companion guide to compare kit characteristics so you can prepare yourself and your lab accordingly.

Electrophoresis of DNA is a fundamental technique in biotechnology that covers a variety of subject material on the structure and function of DNA. Carolina makes the study of electrophoresis attainable for any classroom by offering a number of kits that include valuable teacher resources.

Use this guide to compare kit characteristics so you can prepare yourself and your lab accordingly. No matter what your needs are, we have the right kit for you. If you already use Carolina kits, you might discover a new kit to try with your students!

Type of Samples # of Samples Restriction Digest Required Type of Enzyme Restriction Type of Stain Equipment Included
Beginner Kits
Exploring Electrophoresis of Dyes dyes 6 none none
Best for teaching: The Principles of Electrophoresis.
Scenario: Use dyes and subject them to electrophoresis to determine their relative size.
Exploring Electrophoresis and Forensics Kit DNA 4 none CarolinaBLU
Best for teaching: The Principles of DNA Fingerprinting.
Scenario: Analysis of crime scene whereby DNA evidence is collected from the crime scene, victim and two suspects.
Exploring Electrophoresis of DNA DNA 3 none CarolinaBLU
Best for teaching: Demonstrate the action of restriction enzymes on DNA.
Scenario: Analyze banding pattern of pre-digested DNA when compared to uncut DNA samples after electrophoresis.
Introductory Gel Electrophoresis dyes 8 none none
Best for teaching: The Principles of Electrophoresis.
Scenario: Use dyes and subject them to electrophoresis to determine their relative size.
Type of Samples # of Samples Restriction Digest Required Type of Enzyme Restriction Type of Stain Equipment Included
Intermediate Kits
Nature’s Dice – A Genetic Screening Simulation DNA 24 lyophilized CarolinaBLU
Best for teaching: Mendelian genetics and inheritance, using molecular biology.
Scenario: Perform and analyze a genetic screen on a fictitious family tree. Discover either an Autosomal Recessive or Sex Linked Trait.
Exploring Restriction Analysis and Electrophoresis of DNA DNA 4 lyophilized CarolinaBLU
Best for teaching: Demonstrate the action of restriction enzymes on DNA.
Scenario: Setup restriction digests of lambda DNA using three different enzymes. Separate the digested samples by electrophoresis and analyze.
Restriction Enzyme and DNA DNA 4 lyophilized CarolinaBLU & GelGreen
Best for teaching: Restriction Analysis of Bacteriophage DNA.
Scenario: Lambda DNA is digested using three separate enzymes and analyzed.
DNA Restriction Analysis DNA 4 wet CarolinaBLU & Ethidium Bromide
Best for teaching: Restriction Analysis of Bacteriophage DNA.
Scenario: Lambda DNA is digested using three separate enzymes and analyzed.
Restriction Mapping of Plasmid DNA DNA 4 none CarolinaBLU
Best of teaching: Restriction Mapping of a Plasmid using DNA fragments.
Scenario: Use restriction analysis to piece together a plasmid map from digested DNA fragments.
PCR Forensics Simulation DNA 6 none CarolinaBLU
Best for teaching: Demonstrating the concepts of DNA fingerprinting and PCR.
Scenario: Use a real world scenario to solve a crime by analyzing two loci in a DNA fingerprint.
Outbreak! Fingerprinting Virus DNA DNA 3 none CarolinaBLU
Best for teaching: DNA Analysis of fictional virus.
Scenario: Play the roles of epidemiologists and identify an unknown virus strain using electrophoresis and fragment analysis.
Restriction Enzyme Cleavage of DNA DNA 3 none CarolinaBLU
Best for teaching: Concepts of electrophoresis and restriction enzymes.
Scenario: Perform an electrophoresis with predigested samples with respective enzymes and analyze results. Determine fragment size, calculate relative mobility and use drylabs in this classic lab.
Fast Gels DNA 6 none CarolinaBLU
Best for teaching: Electrophoresis in 15 minutes, using a real world scenario!
Scenario: Choose from two impactful scenarios: Fish DNA fingerprinting or Colon Cancer Testing.
Type of Samples # of Samples Restriction Digest Required Type of Enzyme Restriction Type of Stain Equipment Included
Advanced Kits
Forensic DNA Fingerprinting DNA 4 wet CarolinaBLU & GelGreen
Best for teaching: Advanced techniques in forensic DNA fingerprinting and analysis.
Scenario: Perform plasmid isolation, restriction analysis and electrophoresis of samples taken from a “crime scene” then compare the DNA profiles for a match.
Restriction Mapping of Lambda DNA DNA 3 wet CarolinaBLU
Best for teaching: Restriction Mapping of Lambda DNA using restriction enzymes.
Scenario: Assemble a map of the lambda virus using fragments of DNA digested with three different restriction enzymes.

3 things to consider before you purchase electrophoresis kit(s)

1. What equipment do I already have available?

Before performing electrophoresis, consider what type of equipment (if any) you have available. Whether you have equipment from our biotechnology line or none at all, we have a kit for your classroom. Look for a checkmark in the “Equipment Included” column.

  • All of the electrophoresis kits we sell are compatible with our biotechnology equipment.
  • Our Exploring Electrophoresis kits include all of the equipment and materials to run an electrophoresis.

2. Which electrophoresis topics do I want to teach?

Our labs cover numerous topics within electrophoresis, including the basic principles of electrophoresis, restriction enzymes, DNA fingerprinting, and PCR. Kits may include a combination of these subjects. Check out the “Best for teaching” recommendation.

3. What skill level are my students?

Consider both skill level and appropriateness of subject matter when choosing a kit. For instance, if your class has loaded undigested DNA and run an electrophoresis, but has not performed a restriction digest, choosing a kit that requires students to piece together a plasmid map from DNA fragments will not be a good fit. A kit that requires you to run pre-digested samples and discuss the action of restriction enzymes on DNA would be an appropriate kit to develop your students’ skills. Look for kits in the appropriate skill level section for your class.

Kit scenarios

You may also want to consider the investigative scenario demonstrated in each kit. Many of our kits offer real-world scenarios. Students can play a forensic scientist recreating a crime scene, an epidemiologist investigating the origin of a virus, or a medical professional tracking heritable diseases, to name a few. Using this criterion can be especially helpful when you’re covering cross-curricular concepts–a forensics class with the crime scene scenario, for instance, or a statistics class with the epidemiological scenario.

Choosing the right type of DNA stain has implications on the equipment you’ll use, and more importantly, your and your students’ safety. Carolina offers ethidium bromide-free alternatives such as the CarolinaBLU™ and GelGreen™ dyes that use a white light box and LED blue transilluminators, respectively.

Check out our free video resources for a how-to guide on preparing and pouring a gel and loading a gel for electrophoresis.

Owl Pellets in the Classroom: Safety Guidelines

The dissection of owl pellets can provide a valuable learning experience for students at all grade levels. The following guidelines will help to ensure that this activity is done in a safe fashion.

Owl pellets contain the remains of small animals that the owl has ingested and can be a source of bacterial contamination. Carolina’s individually wrapped owl pellets are heat sterilized at 250º F for 4 hours to eliminate most bacteria, including salmonella bacteria. We do not treat them with chemicals. Keep them wrapped until time to use, to prevent insect infestations or contamination.

Supervise dissection activities.

A teacher or other adult(s) must oversee students’ owl pellet dissection activities to ensure that they perform the activities safely.

Complete dissection activities in one day.

Afterwards, promptly dispose of the owl pellets, plus all disposable materials used in the activity, and remove them from the classroom.

Handle owl pellets, even sterilized ones, as though they could be a source of bacterial or viral contamination.

This is good advice for any and all lab work involving biological materials. The students should learn the importance of good laboratory practices. It will serve them well throughout their academic career and beyond. Caution in the form of good laboratory practices is of great importance whenever one is working with biological materials or chemicals.

Do not use food consumption areas for owl pellet activities.

Owl pellets are not to be dissected in school cafeterias or other food consumption areas. Covering laboratory or classroom tables with an impermeable, disposable material such as aluminum foil will greatly diminish the likelihood of microbial infections. Also use disposable trays, paper, or plates as work surfaces for dissection of the pellets, and dispose of them promptly upon completion of the activity.

No eating or drinking in the dissection area!

During the activity, students must not be allowed to use drinking fountains or get water from sinks for drinking. Eating and drinking should take place before the activity or after the student has completed the activity and thoroughly washed his or her hands, and should take place outside the dissection area.

Use gloves and wash hands.

Give students disposable gloves and instruct them in how to use the gloves properly. Students should also be instructed to keep their hands away from their faces during the activity, and not to touch other surfaces and items away from the work surface and materials. Students should be shown that, when removing gloves, they must avoid skin contact with the exterior of the glove. Common practice is:

  1. Remove the first glove by grasping the cuff, taking care not to touch bare skin, and peeling the glove off the hand so that the glove is inside out.
  2. Remove the second glove while holding the inside of the first in the ungloved hand.
  3. Drop both into the disposal receptacle.

Immediately after the activity and after glove removal and disposal, students must thoroughly wash their hands with soap and warm water, rubbing them with lather for at least 20 seconds before rinsing (as recommended by the U.S. Centers for Disease Control and Prevention—see http://www.cdc.gov/cleanhands), and should dry them with clean paper towels. Be sure sinks are available and well stocked with soap and paper towels. A good antimicrobial foaming skin cleanser for use with water is BactoShield® by Steris. A waterless hand sanitizer can also be a very effective antimicrobial agent if it is comprised of at least 70% alcohol. This can be used in addition to hand washing or, if soap and water are simply not available, in lieu of hand washing (though hand washing is preferable). Do not use a waterless hand sanitizer that does not have this high alcohol content.

Owl pellet dissection provides a good opportunity to teach skills that will serve students well in their academic careers and, for some, in their professional careers or in volunteer activities. They can learn the importance of, and how to use, personal protective equipment (gloves in this instance) and how to protect themselves from microbial infections. Note: Latex gloves can cause allergic reactions in some individuals. Carolina recommends disposable vinyl exam gloves (such as Carolina’s items 706348, 706349, or 706350), or, for this activity with sterilized owl pellets, polyethylene gloves may be used (such as Carolina’s items 706345, 706346, or 706347).

Collect dissection tools, trays, etc., immediately after the activity.

Allow students to use only the tools provided; do not allow them to use pencils or other personal items that they will maintain in their possession after the activity. If possible, use disposable dissection tools and then throw them away. Otherwise, immediately sanitize tools using a bactericidal and virucidal cleaning agent according to its instructions, or by soaking the tools for 2 hours in a 10% household bleach solution or in 70% ethanol.

Wash and sanitize work surfaces immediately after the dissection activity.

Use a cleaning agent that is bactericidal and virucidal, and use according to label instructions. Alternatively, 70% ethanol may be used (be aware that it is flammable), or a 10% household bleach solution makes an effective sanitizing solution (be aware that chlorine bleach is corrosive and irritating to skin and may damage clothing). Use disposable paper towels and throw them away. Do not use sponges or rags that might hold and spread bacteria or viruses. After the students have washed their hands, sanitize the sinks and surrounding surfaces.

Owl pellet dissection is a safe and rewarding activity.

Through this investigation students learn about the food chain and the diets of owls. This activity also provides an opportunity to learn about safe laboratory practices and the importance of taking precautions. We want to reiterate that the owl pellets sold by Carolina have been heat sterilized. This would be expected to eliminate the risk of microbial infections such as salmonellosis. Just as is always advised when working with microbes in the lab, though, we need to assume that owl pellets could be infectious. We need to use good and prudent safety practices to minimize the possibility of any sort of microbial infection. Using sterilized owl pellets and enforcing safe practices make owl pellet dissection a safe and rewarding activity.

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