Description
Intermediate – Easy to perform; requires some background knowledge.
Exploring electrophoresis of DNA classroom kit. Students separate mixtures of precut DNA fragments using samples of lambda DNA—uncut, cut with EcoRI, and cut with HindIII—to effectively demonstrate the action of restriction enzymes and the principles of electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with 1 battery. Classroom Kit includes instructions, 5 sets of apparatus (with 4-well comb), and sufficient materials for 10 experiments.