Description
Intermediate – Requires some background knowledge.
This kit module is great for teaching:
- The molecular basis of heredity
- The relationship between genes and proteins
- How E. coli is used to engineer proteins useful in medicine and manufacturing
- Three techniques – transformation, protein purification, and protein gel electrophoresis – commonly used in biological research and its areas of application
This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 2 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).
In this activity students take their successfully transformed bacteria and use HIC resin to conduct a batch purification technique. This purification process doesn’t require the time or expense typically associated with column-based purification protocols; instead, it produces quick results in 1 class period (40 minutes).
Note: Kit ships with the perishable materials included.