Description
Intermediate—Easy to perform; requires some background knowledge.
Lambda Amplification and Electrophoresis Kit with CarolinaBLU® Stain and 0.2-mL Tubes (with voucher) Good for beginning PCR study. Students amplify a 1,106-bp sequence from the bacteriophage lambda genome using a sample of dilute lambda DNA mixed with PCR Ready-to-Go Beads™ and lambda PCR primers. The mixture can be amplified using 2 water baths (55° C and 100° C) or by using a DNA thermal cycler. Students load the PCR samples onto a 1% agarose gel, electrophorese, and stain. They then verify the size of the amplified product by comparing it to DNA size markers.
This kit also can be used to do time-course studies in which the number of amplification cycles is varied. Students can then observe a qualitative difference in the intensity of the bands on the gel in response to the number of amplification cycles performed.
Kit contains instructions and materials sufficient for 25 amplification reactions or 6 to 8 time-course experiments and gel electrophoresis using CarolinaBLU®.
Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.