DNA Barcode Amplification and Electrophoresis Kit with GelGreen® Stain (with voucher)

Product Code: 211387

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Revised protocol saves you time and delivers robust results
  • Use DNA barcodes to identify student-collected samples of plants or animals
  • Designed, tested, and updated with the classroom in mind
  • Connect students to real-world bioinformatics
  • Identify diversity by taking taxonomy to the next level

This kit explores an emerging trend in molecular biology and brings bioinformatics to the classroom in an accessible manner. Students take a new approach to taxonomy using “DNA barcodes”—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Unlock the barcodes of even more organisms by purchasing the Fungal Primer Set (item #211388, sold separately) to give students the opportunity to explore the diverse world of fungi. Using polymerase chain reaction (PCR) on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences (amplicons) are then submitted for sequencing and can be analyzed using our custom-designed online tool, DNA Subway. Our partner, GENEWIZ, Inc., offers an educational discount for sequencing services.

Kit contains enough materials for 25 DNA extractions, 25 PCR reactions, and running and staining gels with GelGreen®.