Description
Advanced – For experienced high school and college classes; requires some basic technical skill.
- Demonstrate to your students the power of silencing a single gene
- Teach about a powerful method for determining gene function
- Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
- Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness
RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene’s mRNA. Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).
Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables.