Description
Product Details
Proteins are essential tools for life. But how does a simple string of amino acids fold into an intricate three-dimensional structure capable of performing a specific task, such as emitting fluorescence?
Using fluorescent proteins as a model, students explore a protein’s primary, secondary, and tertiary structures and relate those structures to protein function in an integrative, hands-on lab. First, students compare different fluorescent protein sequences at the primary, secondary, and tertiary levels and use that analysis to predict protein functions. Students then express these fluorescent proteins using a simple cell-free protocol to test their functional predictions. This activity serves as an excellent interactive introduction to the basics of protein structure and function, as well as a springboard for discussing more advanced topics such as protein engineering.
Note: These reagents require refrigerated storage. BioBits® pellets are best used within 3 months of receipt when stored at 4° C.
Product Features
- Kit provides enough materials for 8 lab groups
- A P51™ Molecular Fluorescence Viewer (item #216321) or a blue light transilluminator with an orange filter is needed but not included